In this study, we aimed to investigate the relationship between toe spreading ability and toe spreading type on the basis of whether the individual had experienced ankle sprain. The targets of the study were 152 university students (304 feet). The measured items were toe spreading ability and toe spreading type. Toe spreading types were selected from among 12 types. When we investigated 152 subjects (304 feet), 51 of 304 feet had experienced ankle sprain. When we compared the span index of the feet of those with and those without experience of ankle sprain, the span index was significantly lower in the feet of those with experience of ankle sprain. When we compared the incidence of ankle sprain by toe spreading type, the incidence of ankle sprain in types 7 and 10 were significantly higher than that in type 5, and the incidence of ankle sprain in type 7 was significantly higher than that in type 9. The muscle strength and flexibility of the toe muscles were believed to be involved in the toe spreading types. Analyzing potential problems from the perspective of toe spreading types, rather than simply evaluating span index, may be useful for preventing the onset and recurrence of ankle sprain.