学術雑誌論文 〔報告〕高松塚・キトラ両古墳からの主要細菌分離株:Bacillus・Ochrobactrum両属分離株の分子系統学的位置

半田, 豊  ,  立里, 臨  ,  佐藤, 嘉則  ,  木川, りか  ,  佐野, 千絵  ,  杉山, 純多

内容記述
Molecular phylogenetic placements of the three major bacterial taxa, i.e., Bacillus cereus group (28 isolates), Bacillus simplex group (36 isolates), and Ochrobactrum spp. (17 isolates), isolated from various samples collected from the stone chamber interior or exterior environment of Takamatsuzuka Tumulus and Kitora Tumulus were conducted based on the sequences of housekeeping genes (i.e.,gyrB for 17 Bacillus isolates,and gyrB and groEL for 3 Ochrobactrum isolates)in addition to 16S rRNA gene sequences (for all 64 Bacillus and 17 Ochrobactrum isolates). Molecular phylogenetic analyses revealed that almost all isolates belonging to both genera Bacillus and Ochrobactrum were assignable to known species (i.e., Bacillus butanolivorans, B. cereus, B. pseudomycoides, B. simplex, B. thuringiensis, B. toyoensis, and Ochrobactrum pituitosum), and it was possible to infer whether these isolates were pathogenic or not. On the other hand, for the remaining Bacillus and Ochrobactrum isolates, which were not identified as known species, further study is necessary to identify accurately whether these are assignable to novel taxa or not.
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