Departmental Bulletin Paper Global gene expression analysis in the liver of mice administered carcinogens and non-carcinogens For the development of a new rapid and reliable carcinogenicity test

古倉, 聡  ,  高木, 智久  ,  水島, かつら

Although much has been elucidated about the mechanism of cellular carcinogenesis, evaluation of the carcinogenicity of substances is still difficult. Since a number of new synthetic materials for drugs and food have been developed, it is necessary to sufficiently evaluate their carcinogenicities.In carcinogenicity tests performed at present, the test substance is administered to rodents for 2 years (their life span) and its carcinogenicity in each organ is evaluated by macroscopic and pathological examinations. Such carcinogenicity tests require considerable time and resources. In this study, we attempted to develop a new carcinogenicity test by global gene expression analysis, which is independent of target organ-specific activity of carcinogens. All food and chemicals are primarily transported to the liver via the portal vein, both of which are independent of the routes of intake. Therefore, we selected the liver as the best organ with which to assess carcinogenicity in gene expression analysis. The present report indicates the possibility that global gene analysis by using DNA microarray in the liver 3 hours after the administration of chemicals could establish a rapid bioassay to identify carcinogens for various organs.

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