学術雑誌論文 The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development

Kang, Sung-Sik  ,  Ofuji, Sosuke  ,  Imai, Kei  ,  Huang, Weiping  ,  Koyama, Keisuke  ,  Yanagawa, Yojiro  ,  Takahashi, Yoshiyuki  ,  Nagano, Masashi

23 ( 3 )  , pp.412 - 415 , 2015-06 , Cambridge University Press
内容記述
The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5-6 embryos/droplet) showed low blastocyst development compared with a control group (25-26 embryos/droplet). However, small and large numbers of embryos (5-6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25-30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.
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http://clover.rakuno.ac.jp/dspace/bitstream/10659/4256/1/S-2015-26_imai.pdf

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