Departmental Bulletin Paper Functional genomics of the Lactobacillus paracasei strain LC-Ikematsu isolated from the Okinawan natural environment
沖縄の自然界から単離したLactobacillus paracasei LC-Ikematsu 株のゲノム解析

Tada, Ipputa  ,  Saitoh, Seikoh  ,  Aoyama, Hiroaki  ,  Shinzato, Naoya  ,  Morita, Konomi  ,  Yamamoto, Norikuni  ,  Ikematsu, Shinya  ,  多田, 一風太  ,  齋藤, 星耕  ,  青山, 洋昭  ,  新里, 尚也  ,  森田, この美  ,  山本, 憲国  ,  池松, 真也  ,  Department of Genetics, SOKENDAI (The Graduate University for Advanced Studies)  ,  Dept. of Bioresources Engineering, National Institute of Technology  ,  Tropical Biosphere Research Center, University of the Ryukyus  ,  Okinawa Ham Savoury Food Company

11pp.1 - 8 , 2017-03-30 , 沖縄工業高等専門学校 , National Institute of Technology, Okinawa College
We performed functional genomics of lactic acid bacteria, Lactobacillus paracasei strain LC-Ikematsu, using two next-generation sequencers 454 GS Junior (Roche) and MiSeq (Illumina). The genome sequences were assembled using a GS De Novo Assembler (Roche), resulting in 87 contigs (>500 bp) and a total contig length of 3,078,383 bp. The assembled genome sequence was predicted to contain many more amino acid metabolism genes than those of the other Lactobacillus species. Reconstruction of the metabolic pathways using the Kyoto Encyclopedia of Genes and Genomes database suggested that LC-Ikematsu could potentially biosynthesize nine amino acids. The amino acid requirements of LC-Ikematsu were examined using various modified versions of the complete chemically defined medium deficient in specific amino acids. Consequently, we elucidated that LC-Ikematsu could synthesize seven amino acids. This study also establishes bioinformatics techniques for the analysis of bacterial characteristics, which could be effectively applied to other microorganisms.

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