Journal Article Comparison between the Traditional Internal Pressure Filtration Method and a Novel External Pressure Filtration Method of Cell-free and Concentrated Ascites Reinfusion Therapy (CART) along with a Comparison of Refractometer and Biuret Methods for Total Protein Concentration Measurement
Comparison between the Traditional Internal Pressure Filtration Method and a Novel External Pressure Filtration Method of Cell-free and Concentrated Ascites Reinfusion Therapy (CART) along with a Comparison of Refractometer and Biuret Methods for Total Protein Concentration Measurement

Masaaki, Hata  ,  Masanori, Kawaguchi  ,  Toshinori, Oda  ,  Ichirou, Nishiyama  ,  Naohiro, Kouno  ,  Kyousuke, Gouda  ,  Kunihiro, Yamahara  ,  Kenji, Hamabata  ,  Department of Clinical Engineering, Saiseikai Wakayama Hospital  ,  Department of Gastroenterology, Saiseikai Wakayama Hospital  ,  Department of Clinical Engineering, Saiseikai Wakayama Hospital  ,  Department of Gastroenterology, Saiseikai Wakayama Hospital  ,  Department of Gastroenterology, Saiseikai Wakayama Hospital  ,  Department of Gastroenterology, Saiseikai Wakayama Hospital  ,  Department of Gastroenterology, Saiseikai Wakayama Hospital  ,  Department of Kidney Center, Saiseikai Wakayama Hospital

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[Purpose] The differences in performance between two filtration methods (the traditional internal pressure filtration method and a novel external pressure filtration method) of cell-free and concentrated ascites reinfusion therapy were investigated. [Methods] This study included a total of seven cases in three patients. Four of these cases showed pale yellow ascites, and three presented with hemorrhagic ascites. Ascitic fluid from each case was divided in half using the principles of vertical fluid pressure variation. Subsequently, traditional or novel filtration was performed in each half, and the amount of filtration, ascites concentration (measured by refractometer), serum total protein, serum albumin concentration, and processing time were compared between the two methods. [Results] The results of comparing traditional and novel filtration methods were : ascites recovery (97.8%±1.3% vs. 97.2%±2.2%), ascites concentration recovery (91.6%±3.9% vs. 91.0%±4.2%), total protein recovery (77.0%±6.2% vs. 76.5%±4.2%), albumin recovery (83.1%±10.7% vs. 85.9%±10.2%), and processing time (14±1.3 vs. 14±1.3 min). Significant differences were not observed in any of the comparisons. Total protein concentration was measured in a total of 35 samples by refractometer and Biuret method, and these two methods showed a strong correlation (correlation coefficient r=0.98). [Conclusions] Differences between traditional and novel filtration methods were not observed in the filtration of ascites derived from cases of hepatic cirrhosis or hepatitis C investigated in the present study. In addition, a strong correlation using both the refractometer and the Biuret method (r=0.98), was observed between the results.
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