Departmental Bulletin Paper 日光老化モデルにおけるコラーゲン分解産物プロリルヒドロキシプロリンの効果―線維芽細胞に対する保護効果からの検討―

森本, 紗貴子  ,  西田, 昌司  ,  Sakiko, MORIMOTO  ,  Masashi, NISHIDA

Epidermal tissue, which consists of fibroblasts and extracellular matrix, supports skin structure and maintains its functions. Collagen is the most abundant protein in extracellular matrix, and activates fibroblasts in epidermis by binding with integrins, cell surface receptors of collagen. However, the amount of collagen decreases upon aging, resulting in the degradation of skin structure and functions such as wrinkles and the slack of skin. It is difficult to supplement collagen directly to aged epidermis, because the size of collagen is large to deliver orally or percutaneously. We hypothesized and transfers to epidermis, could activate fibroblasts and maintain aged skin in gealthy status.After exposure to hydrogen peroxide (0.5mM) to mimic photoaging, mouse fibroblast cells (3T6) slowed decrease in mitochondrial aerobic metabolism adn membrane potentialtogether with reduced cellular proliferation and increased cell death by apoptosisi. In this model, PHP (0~2mM) increased mitochondrial membrane potential and decreased apoptotic cell death dose-dependently, although it showed no significant effects on mitochondrial metabolism and cellular proliferation. Glycyl-proline, a dipeptide also seen in collagen, did not alter 3T6 damages due to the exposure to hydrogen peroxide. These results suggest that PHP improve epidermal structure and functions via the stereospecific binding to integrins and the partial activation of mitochondria, and is useful for the substitution therapy of skin photoaging.

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