Characteristic comparison of wild black-Aspergillus isolated from field with commercial
秋田, 修 ,
大城, 衣賀 ,
伏木, 愛香 ,
境野, 佑阿部, 真紀
実践女子大学生活科学部紀要 = Bulletin of Jissen Women's University Faculty of Human Life Sciences
44 , 2017-03-10 , 実践女子大学
Aspergillus luchuensis and A.luchuensis mut. kawachii (=A.kawachii) are used for koji-making in Japanese distilled liquor “awamori” and “shochu” production. The origin of these domesticated strains is thought to be a contaminant of wild black-Aspergillus from natural environment. We isolated black-Aspergillus from a fields. Isolated strains were identified as A.tubingensis (=A.saitoi) by the genomic structure analysis. Thai rice koji was prepared using two isolates and four commercial strains. The characteristics such as acidity, citric acid content, amount of myceliums of koji made by isolates were not remarkable different compared with that of A.luchuensis and A.kawachii. The activities of enzymes (α-amylase, acid tolerant α-amylase, glucoamylase, neutral protease, acid protease and acid carboxypeptidase) in koji of isolates were slightly lower than that of commercial strains. From these results, it is estimated that the present commercial A.luchuensis strains originated from black-Aspergillus living in the natural environment.