Departmental Bulletin Paper 乳酸菌が脂質代謝に与える影響についての 培養腸管細胞Caco2を用いた検討
The effect of Lactobacillus acidophilus strain L-55 on chylomicron apoB48 synthesis and secretion by cultured intestinal cells

冨重, 慶子  ,  松島, 照彦

Postprandial hyperlipidemia has been noted as a risk factor in cardiovascular diseases and cell biological studies targeted on intestinal tract is important for the analysis of the secretion of chylomicron. Meanwhile, preventive potentials for atherosclerosis were reported in many functional foods and food components. In order to elucidate the effect of lactic acid bacteria on chylomicron metabolism, we investigated the secretion of apoliporotein B48 (apoB48) in cultured intestinal cells. Human intestinal cell line Caco-2 cells were cultured on the pored membrane of double-based dishes and Lactobacillus acidophilus strain L-55 was added with lipid micelles to the upper chamber. We established an anti-apoB-48 monoclonal antibody, and apoB-48 secreted into the medium was quantified by ELISA. By the addition of L-55, apoB48 secretion was significantly decreased as compared to the control. There was no difference in the decrease between living cells and the heat-killed cells. However, there was a difference in the amount secreted by the timing of the addition and the number of lactic acid bacterium. An observed reduction in the secretion of apo B48 is considered to indicate a decrease in the chylomicron particle numbers. It was suggested that Lactobacillus L-55 may be useful in the control prevention of arteriosclerosis.

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