This study aimed to examine the relationship between low back pain and stressors in terms of care workers' professional qualifications. The participants in this study were 295 care workers, who were surveyed using the Visual Analog Scale (VAS), Roland-Morris Disability Questionnaire (RDQ), and Brief Scales for Job Stress (BSJS). We carried out an inventory survey and analyzed the data using chi-square test and Kruskal-Wallis test. The incidence of low back pain was more than 70% in care workers. There were no significant differences in the incidence of low back pain among the three care worker groups, and low back pain was related to psychological stress. The levels of psychological stress were significantly higher in the care worker group that attended training school than that in other care worker groups. In addition, they were related to low back pain and job satisfaction. The results suggest there were no significant differences in low back pain in the three care worker groups. However, there were significant differences in the factors correlated to low back pain in the three care worker groups.