Journal Article Identification of Carnitine Transporter CT1 Binding Protein Lin-7 in Nervous System
Identification of Carnitine Transporter CT1 Binding Protein Lin-7 in Nervous System

Kenji, Hanada  ,  Takahiro, Nakata  ,  Motoshi, Ouchi  ,  Misaki, Ohtsubo  ,  Makoto, Isono  ,  Asuka, Morita  ,  Kazuhisa, Okamoto  ,  Naoyuki, Otani  ,  Yasushi, Kobayashi  ,  Naohiko, Anzai  ,  Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine  ,  Department Of Anatomy And Cell Biology, Tokoha University:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Health Science, Ishikawa Prefectural Nursing University:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Anatomy And Neurobiology, National Defense Medical College:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Anatomy And Neurobiology, National Defense Medical College:Department Of Health Science, Ishikawa Prefectural Nursing University  ,  Department Of Pharmacology And Toxicology, Dokkyo Medical University School Of Medicine:Department Of Health Science, Ishikawa Prefectural Nursing University

43 ( 1 )  , pp.31 - 38 , 2016-03-25
ISSN:03855023
NCID:AA00629581
Description
_L-Carnitine is an essential component of mitochondrial fatty acid b-oxidation in the muscle and may control the acetyl moiety levels in the brain for acetylcholine synthesis. Carnitine transporter 1(CT1)is the high affinity _L-carnitine transporter whose localization was observed in the kidney, testis, liver, skeletal muscle and brain. To clarify the molecular mechanism of carnitine transport, we sought to find the interacting protein that may be related to the transport function of CT1. Using the intracellular C-terminal region of rat CT1 containing PDZ(PSD95/DLG/ZO-1)motif as bait, we performed the yeast two-hybrid screening against rat brain cDNA library. Thirty two positive clones were obtained from the 2.7×10^7 clones screened. One of them was PDZ domain-containing protein Lin-7. We found that Lin-7 interacts specifically with C-termini of CT1:deletion and mutation of the CT1 C-terminal PDZ-motif abolished the interaction with Lin-7 in the yeast two-hybrid assay. In addition, a PDZ domain within Lin-7 associates with the CT1 C-terminal. The association of CT1 with Lin-7 enhanced _L-carnitine transport activities in HEK293 cells although there is no statistical significance. Coexpression of Lin-7 and CT1 is identified in motor neurons of the spinal cord ventral horn together with Lin-2, a binding partner of Lin-7 known to assemble proteins involved in synaptic vesicle exocytosis and synaptic junctions. Therefore, Lin-7 interacts with CT1 and may regulate their subcellular distribution or function in central nervous system.
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