The purpose of this study was to investigate the effect of low intensity bicycle ergometer exercise at different pedal rates on local muscle oxygenation. Eight healthy male subjects participated in the study. Subjects performed a bicycle ergometer exercise for 6 min at 80 W. Pedal rate was selected as 40, 80, and 120 rpm. We measured cardiorespiratory responses, vastus lateralis muscle activity, and vastus lateralis muscle oxygenation using near-infrared spectroscopy. Oxygen intake and vastus lateralis muscle activity at a pedal rate of 120 rpm were higher than that for 40 rpm and 80 rpm. total Hb in the vastus lateralis was not different between the three pedal rates. At 120 rpm pedal rate, oxy Hb in the vastus lateralis increased significantly, while deoxy Hb in the vastus lateralis decreased significantly, indicating that local muscle oxygen uptake was increased. These data suggest that the increase in muscle activity and local muscle oxygen uptake at a high pedal rate may lead to increased oxygen intake at the same exercise intensity.