||Deletion of TLS polymerases promotes homologous recombination in Arabidopsis
坂本, 綾子 ,
Kaya, HidetakaEndo, Masaki
Plant Signaling & Behavior
2018-06 , Taylor & Francis Online
To investigate how TLS activities are regulated and how the stalled replication fork is processed in plants, reversion frequencies and homologous recombination (HR) frequencies were analyzed using GUS-based substrates. The HR frequencies in plants deficient in DNA polymerase ζ or Rev1 were higher than that in wildtype plants under normal conditions. To examine the role of HSP90 in the regulation of damage tolerance, we analyzed reversion frequencies and HR frequencies in plants grown in the presence of a HSP inhibitor, GDA. Reversion frequency was lower in GDA-treated plants than controls. The HR frequency was higher in GDA-treated wildtype plants than controls. These results suggest that HSP90 interacts with TLS polymerase(s), which promotes error-prone TLS in plants.