Presentation PET-guided surgery system with 64Cu-PCTA-cetuximab for accurate resection of intraperitoneal tumors in a mice model

Yoshii, Yukie  ,  Tashima, Hideaki  ,  Iwao, Yuma  ,  Takuwa, Hiroyuki  ,  Yoshida, Eiji  ,  Wakizaka, Hidekatsu  ,  Yamaya, Taiga  ,  Yoshimoto, Mitsuyoshi  ,  Matsumoto, Hiroki  ,  Ming-Rong, Zhang  ,  Sugyo, Aya  ,  Tsuji, Atsushi  ,  Higashi, Tatsuya

2018-05-31
Description
Cytoreductive surgery is widely used for the treatment of peritoneal dissemination in clinical practice. However, it is difficult to detect and remove tumors that are located deeply in the peritoneal cavity and easily changing the position during an operation. We have found that a PET probe 64Cu-PCTA-anti-EGFR antibody (cetuximab) highly accumulates in intraperitoneal tumors by intraperitoneal administration. Meanwhile, we have developed the world's first open-type PET system, called "OpenPET". In this system, the detectors were arranged to make open space sufficient enough for surgical procedures. In addition, it can image and track objects with high-resolution PET in real-time. In this study, we examined a feasibility of an OpenPET-guided surgery with 64Cu-PCTA-cetuximab to detect and remove intraperitoneal tumors. Methods: Human colon cancer HCT116 cells stably expressing RFP were intraperitoneally seeded into mice. At 1 week after cell inoculation, 64Cu-PCTA-cetuximab (7.4 MBq / mouse) was administered intraperitoneally. At 24 h later, the OpenPET-guided surgery was performed with a small prototype of the OpenPET. Results: The OpenPET-guided surgery clearly detected tumors located deeply in the peritoneal cavity. Measurement time to accumulate sufficient data to identify the tumors was about 10-30 s. We resected the tumors (≥ 3 mm in diameter) located deeply in the peritoneal cavity while monitoring them with the OpenPET real-time imaging. OpenPET was useful to confirm the presence or absence of residual tumors during an operation. Conclusions: We developed an OpenPET-guided surgery with 64Cu-PCTA-cetuximab to detect and remove intraperitoneal tumors located deeply in the peritoneal cavity. This method could provide a novel strategy of cytoreductive surgery for the patients with peritoneal dissemination from gastrointestinal cancers.
第 13 回日本分子イメージング学会総会・学術集会

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