||Hyperpolarized [1-13C]pyruvate MRS reveals early-phase transition of energy metabolism in multicellular spheroid tumors
Takakusagi, Yoichi ,
Takakusagi, Kaori ,
Inoue, KaoriIchikawa, Kazuhiro
Most tumor cells rely on aerobic glycolysis instead of mitochondrial oxidative phosphorylation (OXPHOS) to generate energy (ATP production) for cellular activities. This distinct glucose metabolism, known as the Warburg effect, is further amplified by an organization of the cells as solid tumor tissues in vivo. However, much has remained elusive; e.g. when and how the metabolic shift is triggered during tumorigenesis. In the present study, hyperpolarized (HP) [1-13C]pyruvate MRS was employed to directly monitor the transition of energy metabolism in tiny multicellular tumor spheroids of early-phase. The HP [1-13C]pyruvate conversion to [1-13C]lactate in the smaller tumor spheroids was noninvasively monitored on an NMR spectra.