学術雑誌論文 Hyperpolarized C Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of l-Lactate in Vivo.

Takado, Yuhei  ,  Cheng, Tian  ,  A M Bastiaansen, Jessica  ,  A I Yoshihara, Hikari  ,  Lanz, Bernard  ,  Mishkovsky, Mor  ,  Lengacher, Sylvain  ,  Comment, Arnaud

内容記述
The dynamics of l-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C magnetic resonance spectroscopy (MRS). Following the intravenous injection of hyperpolarized [1-C]lactate, we observed that the distribution of the C label between lactate and pyruvate, which has been shown to be representative of their pool size ratio, is different in NMRI and C57BL/6 mice, the latter exhibiting a higher level of cerebral lactate dehydrogenase A ( Ldha) expression. On the basis of this observation, and an additional set of experiments showing that the cerebral conversion of [1-C]lactate to [1-C]pyruvate increases after exposing the brain to ultrasound irradiation that reversibly opens the BBB, we concluded that lactate transport is rate-limited by the BBB, with a 30% increase in lactate uptake after its disruption. It was also deduced from these results that hyperpolarized C MRS can be used to detect a variation in cerebral lactate uptake of <40 nmol in a healthy brain during an in vivo experiment lasting only 75 s, opening new opportunities to study the role of lactate in brain metabolism.

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