||Analysis of radiation-induced mutation by focusing on plant pigment synthesis genes
平田, 翔也 ,
北村, 智 ,
佐藤, 勝也 ,
Narumi, Issay大野, 豊
QST Takasaki Annual Report 2016
p.94 , 2018-03
Ion beams are useful tools to induce mutations in plants and microorganisms and produce valuable varieties. By analyzing characteristics of ion-beam-induced mutations, we could systematically understand what type of mutation is induced under certain conditions and what is the best condition to obtain mutants efficiently. If the factor that efficiently causes a specific mutation is clarified, more effective ion-beam breeding system could be established. In this work, we focused on plant pigment (anthocyanin) synthesis genes and developed an experimental system to investigate the characteristics of mutations induced by ion-beams. Two anthocyanin synthesis genes (TT4 and TT8), former is expressed constitutively in whole developmental stage but the latter is expressed specifically in reproductive developmental stage, are chosen as targets of the experiment in order to address the relationship between gene expression and mutation frequency.