||Low Cesium-accumulating Mutants of Rhodococcus erythropolis CS98 Generated by Ion Beam Breeding
佐藤, 勝也 ,
Ozawa, Shogo ,
Hayashi, Hidenori大野, 豊
QST Takasaki Annual Report 2016
Rhodococcus erythropolis CS98 was isolated from soil as a cesium (Cs)-accumulating bacteria. However, the mechanisms of Cs-accumulation in R. erythropolis CS98 are still incompletely understood. In this study, we tried to generate mutants that altered Cs-accumulating ability by ion beam breeding technology. Freeze-dried cells were irradiated with carbon ion beamor with gamma rays. Candidates of altered Cs-accumulating mutants were screened from the mutagenized cells using the cell growth under the high Cs concentration as an index. The Cs concentration in the candidates was measured with an atomic absorption spectrometer. As a result, we screened 7 candidates, that were exhibited lower intracellular Cs concentration than that of the parental strain, and in particular 2 candidates were over 1/5-fold lower.