一般雑誌記事 Mutational Breeding of Salt-Resistant Chlamydomonas sp. Strains Reveals Salinity Stress-Activated Starch-to-Lipid Biosynthesis Switching

Kato, Yuichi  ,  Shih-Hsin, Ho  ,  Ogino, Chiaki  ,  Hasunuma, Tomohisa  ,  佐藤, 勝也  ,  大野, 豊  ,  Kondo, Akihiko

内容記述
High salinity stimulates lipid accumulation in the green microalga Chlamydomonas sp. JSC4 by activating a starch-to-lipid biosynthesis switching mechanism. The aim of this study was to improve JSC4 algal biomass production under high salinity conditions by breeding a salt-resistant strain. Microalgal cells seeded on agar plates were irradiated with 50 Gy of the carbon ion beams at TIARA. After long-term and continuous cultivation with high salinity, the salt-resistant strain (KHI) that grow well even in the presence of 7% sea salt was successfully obtained. Under 7% sea salt, the KHI showed the biomass productions, while that of JSC4 did not. The KHI showed higher starch and lipid contents under 7% sea salt, , while that of JSC4 also did not. These results suggest that high salinity stress, not the salinity condition itself, is important for activating lipid accumulation mechanisms in microalgae.

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