Article Screening of Mutants Generated by Heavy Ion Beam for Identification of Genes Involved in Bacterial Interaction

Yanagisaw, Masaomi  ,  Asamizu, Syumpei  ,  Sugai, Yoshinori  ,  佐藤, 勝也  ,  大野, 豊  ,  Onaka, Hiroyasu

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Actinomycetes are filamentous, gram-positive bacteria that are prominent producers of bioactive natural metabolites, which can serve as drug candidates. The aim of this study was to elucidate the genetic factor(s) that are involved in the activation of signaling pathways of secondary metabolism within actinomycetes. In this study, we aimed to generate a mutant library of Streptomyces coelicolor (Sco) to screen for mutants that had lost the ability to produce red pigment (RED) upon Tsukamurella pulmonis (Tp) induction. We first examined the survival rates for carbon ion beams. Then, we observed a dose-dependent decrease in survival rates. We next screened for mutants by mixing the spores with the Tp culture on an agar plate. Then, from the 131 mutants of Sco,we found that 8 mutants were able to produce RED induced by osmotic stress.

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