Article Characterization of Sinorhizobium Mutants Showing High Salt Tolerant Using the Ion Beam Mutation Breeding

Maruyama, Yudai  ,  Tomooka, Norihiko  ,  佐藤, 勝也  ,  大野, 豊  ,  Yokoyama, Tadashi

Vigna marina is one of wild legumes, which called as Hamaazuki, are distributed at the vicinity of the coast from tropical to sub-tropical regions and Vigna marina can grow at 400 mM NaCl condition. Furthermore, we found that the root nodules on Vigna marina have a symbiotic relationship with rhizobia that related to the genus Sinorhizobium having high stress tolerance (500 mM NaCl, 45 ºC, pH 10.5). In this study, to improve symbiotic performance of Vigna marina with Sinorhizobium under high salt conditions, we attempted to enhanced salt tolerant ability using ion beam mutation breeding. Consequently, we could obtain 5 mutants having high NaCl tolerant which can survive at 7.5% NaCl concentration. Of the 5 mutants, 2 mutant could survive at 8% salt containing media, however remaining 3 mutants could not survive at the condition for 3 weeks.

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