||miRNA 200c Sensitizes Pancreatic Cancer Stem Cells to Carbon Ion Beam Irradiation
崔, 星 ,
鈴木, 雅雄Eun Ho, Kim
In the present study, we investigate new molecular mechanisms produced by carbon ion beam in combination with miRNA200c mimic from the point of view of targeting CSCs in vitro. Human pancreatic CSCs sorted from PK45, PANC1 cells were treated with carbon ion beam, X-ray irradiation alone or in combination with miRNA200c mimic, and then cell viability assay, colony, spheroid formation ability assay, and real time PCR analysis of apoptosis and autophagy related gene expression were performed. Carbon ion beam combined with miRNA200c mimic significantly decreased CSC viability. The colony, spheroid formation assays confirmed that subpopulation of CD44+/ESA+ exactly have CSC properties compared to CD44-/ESA- cells in pancreatic cancer cells. Real time PCR analysis showed that some apoptosis- and autophagy-related gene expression was significantly induced after carbon ion beam combined with miRNA200c mimic in pancreaticCSCs. Taken together, carbon ion beam has superior potential to kill pancreatic CSCs when combined with miRNA200c mimic compared to carbon ion beam alone.
The 16th edition of the International Congress on Targeted Anticancer Therapies (TAT)