||A method for imaging of uranium distribution on rat kidney sections by detection of α-particle tracks using CR-39 plastic nuclear track detectors.
Konishi, Teruaki ,
Kodaira, Satoshi ,
Itakura, Yuichi ,
Ohsawa, DaisukeShino, Homma-Takeda
Uranium (U) is known as a global contaminant and is a major concern for health risk due to its high toxicity. In this study, our aim was to develop a possible simple method to identify accumulated regions or localized sites of U in kidney using CR-39 plastic nuclear track detector. To demonstrate our method, the renal cryo-sections (5 μm-t) from Wistar male rats (10 weeks old) administered with U-acetate (2 mg/kg) subcutaneously were prepared on day one after administration. The section were placed on CR-39, stored for one year and then etched in a 7 M NaOH solution at 70 ° C for 3 hours to visualize the α-tracks. Addition to the detection of α-tracks, the difference of UV light absorption of tissue structure affected the bulk etch velocity resulted surface roughness of CR-39 that represented tissue structure.As a result, total of 2449 α-tracks were detected in analyzed area of 144 mm2. The cortex (CO), the outer stripe of the outer medulla (OSOM), and the medulla were measured to be 77.2 mm2, 39.4 mm2, 22.7 mm2 from the HE-stained image and were identical with the transferred tissue relief on the CR-39. Number of α-tracks detected in CO and OSOM were 345 and 1986, indicating that the accumulation of U was nearly 6-fold higher in OSOM than CO. As conclusion, this method made possible to simultaneously identify the U accumulation region in the tissue section depending on the trajectory of α-tracks.
Micros 2017 - 17th International Symposium on Microdosimetry