Journal Article Synergistic Effects of Arsenite on Radiosensitization of Glioblastoma Cells

二宮, 康晴  ,  于, 冬  ,  関根, 絵美子  ,  中島, 徹夫

37 ( 8 )  , pp.4111 - 4117 , 2018-05 , International Institute of Anticancer Research
BACKGROUND/AIM:Glioblastoma is a frequent type of brain tumor and is radioresistant. Arsenite, which crosses the blood-brain barrier, shows synergistic effects with radiation in vitro and in vivo. The mechanism remains unclear.MATERIALS AND METHODS:As synergistic radiosensitization has been reported in p53-deficient cancer cells, radiosensitization was evaluated using the glioblastoma cell line, U87MG-E6, which harbors inactivated p53, in comparison with the cell line, HCT116 p53 (-/-). Radiosensitivity was evaluated using clonogenic assays and detection of abnormal amplification of centrosomes (AAC).RESULTS:Synergistic effects of arsenite on radiosensitivity were observed in both cell lines. The radiosensitization induced by arsenite was abolished by N-acetyl-l-cysteine, a reactive oxygen species (ROS) scavenger. Increased radiosensitivity by arsenite was also abolished following knock-down of BRCA2. In addition, the increased radiosensitization by arsenite was correlated with AAC, which was abolished by BRCA2 knock-down.CONCLUSION:We conclude that radiosensitization by arsenite is related to ROS and BRCA2 function.

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