Presentation Bystander effects from carbon-ion irradiated tumor to non-irradiated normal cells, simulating spot scanning irradiation

鈴木, 雅雄  ,  横田, 裕一郎  ,  崔, 星

Communication between irradiated tumor and non-irradiated normal cells is one of the important concerns for radiotherapy. This year, we examined biological effects induced in non-irradiated normal cells, focusing on the communication of signaling events from carbon-ion irradiated tumor to non-irradiated normal cells. Human glioblastoma cells (T98G) were irradiated with carbon-ion microbeams generated with the TIARA in QST (LET=103keV/µm), simulating the spot scanning system. The normal human fibroblasts were co-cultured with irradiated T98G cells in the presence or absence of a gap-junction inhibitor using the transwell permeable support system. The carbon-ion irradiation in the absence of the gap-junction inhibitor resulted in increased cell death and gene mutation in the non-irradiated bystander normal cells. In case of the presence of the inhibitor, no differences were observed in both biological endpoints. There is clear evidence that the irradiated tumor cells enable to induce damage in the neighboring non-irradiated normal cells via the gap-junction mediated bystander effect. (This is the co-operative study with Drs. T.Funayama, M.Suzuki and Y.Kobayashi.)

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