Presentation Development of the ex vivo spermatogenesis technique for advanced radiotherapeutic science

Fukunaga, Hisanori  ,  Kaminaga, Kiichi  ,  Sato, Takuya  ,  T. Butterworth, Karl  ,  Yokoya, Akinari  ,  Ogawa, Takehiko

Modern radiotherapeutic techniques can change beam shape and intensity as the machine of a radiation source moves around the patient body to match the shape of the tumor. These advancements have significantly contributed to better clinical outcomes for the tumor; however, they have also dramatically complicated the risk assessments of the surrounding normal tissues due to the heterogeneous radiation dose distribution. In this study, we develop an ex vivo mouse testis culture as a model to determine the biological effects on the germ stem cells and spermatogenesis from the exposure to such modified radiation fields.This organ culture method, using the Acr-GFP transgenic mice, is useful for observing the process of spermatogenesis because the start of meiosis can easily be detected by the GFP expression in the mice testis. The testes were obtained from 7 days postpartum mice, and then they were transferred to an X-ray irradiation facility and cultured for more than one month. We observed in real time and analyzed the radiation-induced impacts on the process of spermatogenesis.Our preliminary data validated our methodology, which accurately reproduces X-ray-induced male germ toxicity, such as temporary infertility and permanent sterility. Furthermore, we have also succeeded in detecting the change in the meiosis process induced by exposure to modified X-ray fields.These results indicate that our model is a robust approach for further investigation of radiotherapeutic toxicity and/or its side effects.

Number of accesses :  

Other information