||Quantitative study of mammalian cells by scanning transmission soft X-ray microscopy
Shinohara, K. ,
Ohigashi, T. ,
Tone, S. ,
加道, 雅孝Ito, A.
IOP Conf. Series: Journal of Physics
012003-4 , 2017-08 , IOP Publishing
Molecular distribution in mammalian cells was studied by soft X-ray scanningtransmission microscopy with respect to the quantitative aspect of analysis. NEXAFS profiles at the C, N and O K-absorption edges were combined and used for the analysis. For the estimation of quantity for nucleic acids and proteins, NEXAFS profiles of DNA and bovine serum albumin (BSA) at the N K-absorption edge were applied assuming that those were their representatives. The method has a potential to explore the other molecular components than nucleic acids and proteins.