||The Importance of the Primary-and Secondary Bystander Responses Cross-Talk between Human Lung Cancer and Lung Normal Cells after Proton Microbeam Irradiation
Autsavapromporn, Narongchai ,
Kobayashi, Alisa ,
Furusawa, YoshiyaKonishi, Teruaki
The radiation-induced bystander effect (RIBE) is an important in clinical particle radiotherapy such as protons and carbon ions because it may increase the probability of normal cellular injury and tumor recurrence that leading to the treatment failure. Particularly, the biological and molecular events underlying the role of gap junction intercellular communication (GJIC) cross-talk between irradiated-cancer and non-irradiated bystander normal cells after protons microbeam irradiation remain largely undefined. In this context, we investigated the role of primary-and secondary bystander response cross-talk between human lung cancer (A549) and normal lung fibroblasts (WI38) cells using Single-Particle Irradiation system to Cell (SPICE) microbeam facility in National Institute of Radiological Sciences (NIRS), Japan (3.4 MeV protons, 2 µm beam diameter, LET 11 keV/µm).Our results suggest that proton microbeams-induced primary bystander responses in both A549 and WI-38 cells. Furthermore, the secondary bystander responses are propagated from the primary bystander A549 cells to non-irradiated bystander WI-38 cells via GJIC detecting by using the transwell insert co-cultured model. In conclusion, these data demonstrated that GJIC play a critical role in mediating protons-irradiation induced primary- and secondary bystander responses, which is directly relevant to cancer radiotherapy.
5th International Symposium on Space Radiation and Particle Radiotherapy