Presentation Comparative exploration of biomarkers in mice exposed to X-ray or Fe-ion radiation

中島, 徹夫  ,  Vares, Guillaume  ,  二宮, 康晴  ,  王, 冰  ,  勝部, 孝則  ,  田中, 薫  ,  劉, 翠華  ,  平川, 博一  ,  丸山, 耕一  ,  藤森, 亮

Biological markers, which indicate radiation effects and are used for predicting late effects in the future, are expected to be identified. The purpose of this study is to explore useful biological markers for evaluating radiation effects.Particularly, low-dose particle radiation may induce bystander effects due to low particle fluence and to compare the changes in molecules after particle irradiation with that after X-rays irradiation might possibly detect particle-radiation specific biomarkers. Metabolic analyses were performed in livers of mice irradiated with X-rays. Dose-dependent or dose-specific alterations were evaluated. In addition to it, investigating differences of biomarkers due to radiation quality, specific alteration in metabolites was compared with that in the case of Fe-ion irradiation. Moreover, we tried to detect inflammatory biomarkers in both X-rays and Fe-ion irradiation.Metabolites in livers of mice (C57BL/6J) were analyzed by capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) one month after X-ray irradiation. Significantly altered metabolites to the control were selected from the irradiation groups and specific trends were evaluated. One month after irradiation, 4, 8 and 16 metabolites were significantly altered in irradiated groups at doses of 0.1Gy, 0.5Gy and 2Gy, respectively, compared to the levels in the control. Significantly altered metabolites included free amino acids. However, changes in free amino acids after Fe-ion irradiation (0, 0.1 and 2 Gy) were not detected. These will be discussed in the viewpoint of amino acid metabolism and usage as biomarkers for evaluating radiation effects with results of cytokine alteration after irradiation.

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