学術雑誌論文 Crystal structure of the overlapping dinucleosome composed of hexasome and octasome

Kato, Daiki  ,  Osakabe, Akihisa  ,  Arimura, Yasuhiro  ,  Mizukami, Yuka  ,  Horikoshi, Naoki  ,  Saikusa, Kazumi  ,  Akashi, Satoko  ,  Nishimura, Yoshifumi  ,  Sam-Yong, Park  ,  Nogami, Jumpei  ,  Kazumitsu Maehara  ,  Ohkawa, Yasuyuki  ,  松本, 淳  ,  河野, 秀俊  ,  Rintaro Inoue  ,  Sugiyama, Masaaki  ,  Kurumizaka, Hitoshi

356 ( 6334 )  , pp.205 - 208 , 2017-04 , AAAS
内容記述
Nucleosomes are dynamic entities that are repositioned along DNA by chromatin remodeling processes. A nucleosome repositioned by the switch-sucrose nonfermentable (SWI/SNF) remodeler collides with a neighbor and forms the intermediate “overlapping dinucleosome.” Here, we report the crystal structure of the overlapping dinucleosome, in which two nucleosomes are associated, at 3.14-angstrom resolution. In the overlapping dinucleosome structure, the unusual “hexasome” nucleosome, composed of the histone hexamer lacking one H2A-H2B dimer from the conventional histone octamer, contacts the canonical “octasome” nucleosome, and they intimately associate. Consequently, about 250 base pairs of DNA are left-handedly wrapped in three turns, without a linker DNA segment between the hexasome and octasome moieties. The overlapping dinucleosome structure may provide important information to understand how nucleosome repositioning occurs during the chromatin remodeling process.

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