||DNA damage response induces structural alterations in histone H3-H4.
Izumi, Yudai ,
Fujii, Kentaro ,
Yamamoto, Satoshi ,
Matsuo, Koichi ,
Namatame, Hirofumi ,
Taniguchi, MasakiYokoya, Akinari
Journal of radiation research
65 , 2017-01 , Oxford University Press
Synchrotron-radiation circular-dichroism spectroscopy was used to reveal that the DNA damage response induces a decrement of α-helix and an increment of β-strand contents of histone H3-H4 extracted from X-ray-irradiated human HeLa cells. The trend of the structural alteration was qualitatively opposite to that of our previously reported results for histone H2A-H2B. These results strongly suggest that histones share roles in DNA damage responses, particularly in DNA repair processes and chromatin remodeling, via a specific structural alteration of each histone.