Journal Article Visualization of complex DNA double-strand breaks in a tumor treated with carbon ion radiotherapy.

Oike, Takahiro  ,  Niimi, Atsuko  ,  Okonogi, Noriyuki  ,  Murata, Kazutoshi  ,  Matsumura, Akihiko  ,  Shin-Ei, Noda  ,  Kobayashi, Daijiro  ,  Iwanaga, Mototaro  ,  Tsuchida, Keisuke  ,  Kanai, Tatsuaki  ,  Ohno, Tatsuya  ,  Shibata, Atsushi  ,  Nakano, Takashi

6pp.22275-1 - 22275-7 , 2016-03 , Nature Publishing Group
Carbon ion radiotherapy shows great potential as a cure for X-ray-resistant tumors. Basic research suggests that the strong cell-killing effect induced by carbon ions is based on their ability to cause complex DNA double-strand breaks (DSBs). However, evidence supporting the formation of complex DSBs in actual patients is lacking. Here, we used advanced high-resolution microscopy with deconvolution to show that complex DSBs are formed in a human tumor clinically treated with carbon ion radiotherapy, but not in a tumor treated with X-ray radiotherapy. Furthermore, analysis using a physics model suggested that the complexity of radiotherapy-induced DSBs is related to linear energy transfer, which is much higher for carbon ion beams than for X-rays. Visualization of complex DSBs in clinical specimens will help us to understand the anti-tumor effects of carbon ion radiotherapy.

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