Presentation Decomposition of averaged EM images by 2D hybrid analysis

松本, 淳

We are developing a computational approach (2D hybrid analysis) to build a 3D atomic model from an electron microscope (EM) image of a biological molecule. In this approach, firstly a lot of atomic models are built by deforming the X-ray crystal structure (or the modeled structure). Then, the projections of each model are created. Finally, they are compared with the EM image to select the best-fitting atomic model. We have recently introduced the realistic projection model (the negative stain model) to improve the accuracy of the computation. This has enabled us to decompose the averaged EM images. In principle, averaging of EM images should be performed using the raw images of molecules with the same conformation and orientation. Actually, mixture occurs. Now we can detect such mixture by the 2D hybrid analysis.

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