Journal Article Inhibitory effects of caffeine on gustatory plasticity in the nematode Caenorhabditis elegans.

Urushihata, Takuya  ,  Takuwa, Hiroyuki  ,  Higuchi, Yukako  ,  Sakata, Kazumi  ,  Wakabayashi, Tokumitsu  ,  Nishino, Asuka  ,  Matsuura, Tetsuya

80 ( 10 )  , pp.1990 - 1994 , 2016-06 , Taylor & Francis
ISSN:0916-8451
Description
The effects of caffeine on salt chemotaxis learning were investigated using the nematode Caenorhabditis elegans. To estimate the degree of salt chemotaxis learning, nematodes were placed in a mixed solution of NaCl and caffeine, and then the chemotaxis index of NaCl was obtained from the nematodes placed on agar medium after pre-exposure to caffeine concentrations of 0.01, 0.1, 0.3, and 1.0%. Locomotor activity and preference behavior for caffeine were also estimated under these caffeine conditions. Nematodes pre-exposed to 0.3% caffeine showed inhibition of salt chemotaxis learning. Additional experiments indicated that nematodes showed a preference response to the middle concentration of caffeine (0.1%), with preference behavior declining in the 0.3% caffeine condition. Stable locomotor activity was observed under 0.01-0.3% caffeine conditions. These results suggest that salt chemotaxis learning with 0.3% caffeine is useful for investigating the effects of caffeine on learning in nematodes.

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