Journal Article Effect of Amifostine, a Radiation-Protecting Drug, on Oxygen Concentration in Tissue Measured by EPR Oximetry and Imaging

Ueno, Megumi  ,  Matsumoto, Shingo  ,  Matsumoto, Atsuko  ,  Manda, Sushma  ,  Nakanishi, Ikuo  ,  Ken-ichiro, Matsumoto  ,  B. Mitchell, James  ,  C. Krishna, Murali  ,  Anzai, Kazunori

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Effect of amifostine, a radiation-protecting drug, on muscle tissue partial pressure of oxygen (pO2) was investigated by electron paramagnetic resonance (EPR) spectroscopy and imaging. When amifostine was administered intraperitoneally or intravenously to mice, the linewidth of the EPR spectra of the lithium octa-n-butoxy-substituted naphthalocyanine (LiNc-BuO) implanted in the mouse leg muscle decreased. EPR oximetry using a LiNc-BuO probe and EPR oxygen mapping using a triarylmethyl-type probe was useful to quantify pO2 in the tissues of living mice. The result of EPR oximetric imaging showed that administration of amifostine can decrease pO2 in the muscle and also tumor tissues. This finding suggests that lowering pO2 in tissues might contribute in part to the radioprotection of amifostine.

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