Conference Paper Abstract 3869: Exploration of mechanisms for chromothripsis by irradiation

森下, 真紀  ,  村松, 智輝  ,  Hayashi, Shin  ,  平井, 百樹  ,  數藤, 由美子  ,  小西, 輝昭  ,  Moriyama, Kenji  ,  Inazawa, Johji

75 ( 15 Supplement )  , pp.3869 - 3869 , 2015-08 , AACR
To explore mechanisms of chromothripsis, we investigated whether chromothripsis was artificially induced by irradiation. We elicited DNA double-strand breaks in the oral cancer cell line HOC313, or its highly metastatic subline HOC313-LM by The Single Particle Irradiation system to Cell (SPICE), a focused vertical micro-beam system designed to irradiate the nuclei of adhesive cells. We established irradiated monoclonal sublines and analyzed the copy number variations (CNVs) and SNPs using SNP array. Chromosomal alterations, induced by irradiation including CNVs, were detected in some monoclonal sublines. Interestingly SNP array analysis showed that one subline named LM200-#25 had multiple chromosomal alterations which happened locally in chromosome 7. Moreover, we detected several chromosomal alterations such as t(12;7;11) translocation in LM200-#25 subline by Multi-Color FISH analysis. These results suggested that chromothripsis might be induced by irradiation in LM200-#25 subline.

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