Presentation 炭素線とゲムシタビンとシスプラチンとの併用による胆管癌幹細胞に対する殺傷効果

Sai, Sei  ,  Vares, Guillaume  ,  Suzuki, Masao

2015-10-09
Description
To investigate whether carbon ion beam alone or in combination with gemcitabine and cisplatin is effective in targeting putative human cholangicarcinoma stem cells in vitro and in vivo, putative cancer stem cells (CSCs) sorted from HuCCT1 and Huh28 cells were treated with carbon ion beam, X-ray alone or in combination with gemcitabine and cisplatin. The number of colony and spheroid formed from CSCs was remarkably reduced, and a more large number of apoptotic cells and γH2AX foci were observed after carbon ion beam combined with gemcitabine and cisplatin compared to that of X-ray or carbon ion beam alone. High dose with 35 Gy alone or with 25 Gy of relative low dose carbon ion beam combined with gemcitabine and cisplatin induced more severe xenograft tumor cell cavitation and fibrosis and significantly regressed compared to that of X-ray combined with gemcitabine and cisplatin. In conclusion, high dose alone or relative low dose of carbon ion beam combined with gemcitabine and cisplatin effectively destroyed cholangicarcinoma stem cells in vitro and in vivo.
第74回日本癌学会学術総会

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