Presentation Cell-cell communication mediated bystander effects between carbon-ion irradiated tumor and non-irradiated normal cells

鈴木, 雅雄  ,  鈴木, 雅雄  ,  崔, 星

Communication between tumor and normal cells is one of the important concerns for radiotherapy. We focused on the communication of signaling events from carbon-ion irradiated tumor to non-irradiated normal cells. Human glioblastoma cells (T98G) were irradiated with carbon-ion beams (LET=73keV/µm) scheduled to either a single dose (6Gy) or three-fraction doses (3x2Gy) during three consecutive days. Then normal human fibroblasts were co-cultured with T98G in presence or absence of a gap-junction inhibitor using the transwell permeable support system. The single-dose irradiation in absence of the gap-junction inhibitor resulted in increased micronucleus formation, cell death and gene mutation in the bystander normal cells. On the other hand, the fraction-dose irradiation showed higher micronucleus formation and mutation than the single dose, but the same level of cell death with the control. In case of presence of the inhibitor, no differences were observed in three biological endpoints. There is clear evidence that the irradiated tumor cells enable to induce damage in the neighboring non-irradiated normal cells via the gap-junction mediated bystander effect.

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