Presentation The New Low-Cost Metaphase Finder and It’s Application for Non-Human Chromosomes

Furukawa, Akira

Metaphase finder is a tool for biodosimetry that finds metaphase chromosomes among blood cells on slide glasses. It consists of automated microscope, auto-focus system, X-Y stage, camera and computer. It does the image diagnosis of the microscopic images of the slide glasses, and displays the positions of metaphase cells. A metaphase finder is used for the personnel worked at Fukushima nuclear plant to know how much dose they irradiated. The author and colleagues have already reported a low-cost metaphase finder system, using commercially-available products. But the system was a combination of multiple manufacturers and then it requires much knowledge and skill to the resellers and users. The system was more compact and less price than commercial products. The system would be another choice for resellers and users, but its speed was needed to faster. Then, the author had collaborated with a software company to make another system. We used Nikon Eclipse Ni-E microscope with motorized X-Y stage, 4x objective lens and 1920 x 1024 pixels camera for hardware. Its software was selected from two different programs. One was written in macro language for ordinal microscope controller, which is shown on the catalogue. The other was the newly written special software to be faster than non-custom made system. This was added the new function to compare the color of the image. We accomplished making the system and tested its speed. It was 13 minutes and 56 seconds per one slide, while capturing 1,333 images for special software. The programs were both application of mathematical morphology, and it is applicable for not only human chromosomes but also non-human chromosomes. This presentation will focus on non-human chromosomes. This research is supported by The Japan Science and Technology Agency.
The 5th Asian Chromosome Colloquium

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