Journal Article Lymph node mapping using quantum dot-labeled polymersomes

Rumiana, Bakalova  ,  Zhelov, Zhivko  ,  Nikolova, Billiana  ,  Murayama, Shuhei  ,  Lazarova, Desislava  ,  Tsoneva, Idana  ,  Aoki, Ichio  ,  Saga, Tsuneo

34 ( 4 )  , pp.393 - 398 , 2015-10 , AEPress
The present study was designed to investigate whether poly-ion complex hollow vesicles (polymersomes), based on chemically-modified chitosan, are appropriate for lymph node mapping in the context of their application in the development of theranostic nanosized drug delivery systems (nano-DDS). The experiments were performed on Balb/c nude mice (colon cancer-grafted). The mice were subjected to anesthesia and quantum dot (QD705)-labeled polymersomes (d~120 nm) were injected intravenously via the tail vein. The optical imaging was carried out on Maestro EX Imaging System (excitation filter: 435–480 nm; emission filter: 700 nm). A strong fluorescent signal, correspondingto QD705 fluorescence, was detected in thelymph nodes, as well as in the tumor. A very weak fluorescent signal was found in the liver area. The half-life of QD705-labelled polymersomeswas 6 ± 2 hours in the bloodstream and 11 ± 3 hours in the lymph nodes. The data suggest that polymersomes are very promising carriers for lymph node mapping using QD as a contrast agent. They are useful matrix for development of nano-formulations with theranostic capabilities.

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