Presentation The project of new low-cost metaphase finder

Furukawa, Akira

Metaphase finder is a tool for biodosimetry that finds metaphase chromosomes among blood cells on slide glasses. It consists of automated microscope, auto-focus system, X-Y stage, camera and computer. It does the image diagnosis of the microscopic images of the slide glasses, and displays the positions of metaphase cells. A metaphase finder is used for the personnel worked at Fukushima nuclear plant to know how much dose they irradiated.The author and colleagues have already reported a low-cost metaphase finder system, using commercially-available products. Its software was application of mathematical morphology. But the system was a combination of multiple manufacturers and then it requires much knowledge and skill to the resellers and users. The system was more compact and less price than commercial products. The system would be another choice for resellers and users, but its speed was needed to faster.Then, the author has collaborated with a software company to start new project to make another system. This system is using new special software is expected to be faster than non-custom made system. We used Nikon Eclipse Ni-E microscope with motorized X-Y stage, 4x objective lens and 1920 x 1024 pixels camera for hardware. The software added the new function to compare the color of the image.Now, the system was completed and the project was finished. We tested its speed, and it was 13 minutes and 56 seconds per one slide, while capturing 1,333 images. We accomplished the aim of the project.This research is supported by The Japan Science and Technology Agency.
ICRR2015 (15th International Congress of Radiation Research)

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