Presentation IWM2015

Konishi, Teruaki  ,  Kobayashi, Alisa  ,  KN Yu, Peter  ,  Yang, Gen  ,  Ahbrizal Farizal Tengku Ahmad, Tengku  ,  Oikawa, Masakazu  ,  Furusawa, Yoshiya

Microbeams made it possible to deliver a defined number of charged particles on a single cell with a resolution of a few micrometers, and are most advantageous for the studies of biological response on low dose radiation. Our microbeam irradiation system, the Single-Particle Irradiation system to CEll (SPICE) provides a 3.4 MeV proton microbeam focused with a quadrupole magnetic lens on an upward vertical beam line. At present, SPICE can target mammalian cells from single ion with stability and high throughput using an upward vertical beam of below 2-micrometer in diameter [1]. A variety of irradiation modes have been established for radiation-induced bystander effects, cytoplasm irradiation etc. Within these several years, we have focused on the radio-induced bystander effects, especially on the aspect on the damaging and protective bystander response and signaling between two different kinds of cells, such as 1) human lung cancer and normal cells [2], and 2) cancer stem-like cells, CSCs and non stem-like cancer cells, NSCCs in human fibro-sarcoma HT1080 cells[3]. We also expanded our studies to bystander effect in vivo using Zebrafish embryos for bystander effect induced adaptive response[4].Keywords: SPICE; proton; microbeam; DNA damage; cytoplasm irradiationREFERENCES1.Konishi, T., Oikawa, M., Suya, N., et al. SPICE-NIRS Microbeam: a focused vertical system for proton irradiation of a single cell for radiobiological research. J Radiat Res, 2013; 54: 736–747.2.Desai S, Kobayashi A, Konishi T, et al. Damaging and protective bystander cross-talk between human lung cancer and normal cells after proton microbeam irradiation. Mut Res, Fund Mol Mech Mut 2014; 763-764: 39-443.Liu Y, Kobayashi A, Maeda T, et al. Target irradiation induced bystander effects between stem-like and non stem-like cancer cells. Mut Res, Fund Mol Mech Mut (in press)4.Choi VWY, Konishi T, Oikawa M, et al. Adaptive response in zebrafish embryos induced using microbeam protons as priming dose and X-ray photons as challenging dose. J Radiat Res 2010; 51: 657–64.
The 12 th International Workshop on Microbeam Probes of Cellular Radiation Response

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