学術雑誌論文 A laser-plasma-produced soft X-ray laser at 89 eV generates DNA double-strand breaks in human cancer cells.

Sato, Katsutoshi  ,  Nishikino, Masaharu  ,  Kawachi, Tetsuya  ,  Shimokawa, Takashi  ,  Imai, Takashi  ,  Teshima, Teruki  ,  Nishimura, Hiroaki  ,  Kando, Masaki

56 ( 4 )  , pp.633 - 638 , 2015-04 , Oxford University Press
ISSN:0449-3060
内容記述
While it has been expected that X-ray laser will be widely applied to biomedical studies, this has not been achieved to date and its biological effects such as DNA damage have not been evaluated. As a first step for its biological application, we developed a culture cell irradiation system, particularly designed for a plasma-driven soft X-ray laser pulse, to investigate whether the soft X-ray laser is able to induce DNA double strand breaks (DSBs) in living cells or not. The human adenocarcimona cell line A549 was irradiated with the soft X-ray laser at a photon energy of 89 eV and the repair focus formation of the DSBs was assessed by immunofluorescence staining with antiphosphorylated DNA-PKcs (p-DNA-PKcs), ATM (p-ATM) and γ-H2AX antibody. The p-DNA-PKcs, ATM, and γ-H2AX foci were clearly identified after soft X-ray laser irradiation. Furthermore, the increase in the X-ray laser shot number, even from a single shot, results in the increase in p-DNA-PKcs foci. These results are the first evidence that the 89 eV soft X-ray laser is able to induce DSB in living cells. Our study demonstrated that this irradiation system is a useful tool for investigating the radiobiological effect of soft X-ray laser.

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