学術雑誌論文 Antibiotic resistance in Escherichia coli strains isolated from Antarctic bird feces, water from inside a wastewater treatment plant, and seawater samples collected in the Antarctic Treaty area

10 ( 2 )  , pp.123 - 131 , 2016-06
ISSN:18739652
内容記述
Antibiotic resistance is a problem of global concern and is frequently associated with human activity. Studying antibiotic resistance in bacteria isolated from pristine environments, such as Antarctica, extends our understanding of these fragile ecosystems. Escherichia coli strains, important fecal indicator bacteria, were isolated on the Fildes Peninsula (which has the strongest human influence in Antarctica), from seawater, bird droppings, and water samples from inside a local wastewater treatment plant. The strains were subjected to molecular typing with pulsed-field gel electrophoresis to determine their genetic relationships, and tested for antibiotic susceptibility with disk diffusion tests for several antibiotic families: β-lactams, quinolones, aminoglycosides, tetracyclines, phenicols, and trimethoprim–sulfonamide. The highest E. coli count in seawater samples was 2400 cfu/100 mL. Only strains isolated from seawater and the wastewater treatment plant showed any genetic relatedness between groups. Strains of both these groups were resistant to β-lactams, aminoglycosides, tetracycline, and trimethoprim–sulfonamide.In contrast, strains from bird feces were susceptible to all the antibiotics tested. We conclude that naturally occurring antibiotic resistance in E. coli strains isolated from Antarctic bird feces is rare and the bacterial antibiotic resistance found in seawater is probably associated with discharged treated wastewater originating from Fildes Peninsula treatment plants.
抗生物質耐性菌は地球規模の問題となっており、しばしば人間活動と関わっている。重要な糞便汚染指標菌である大腸菌を、南極でも人間活動の影響の強いキングジョージ島のFildes半島の海水、鳥の糞、基地の汚水処理施設内の水から単離し、分子タイピングにより遺伝的関係を決定し、数種の抗生物質に対する感受性が調べられた。海水と汚水処理施設からの株の間では遺伝的関連が無く、これらの株では、数種の抗生物質に対して耐性があった。対照的に鳥の糞からの株は、全ての抗生物質に対して耐性を持たなかった。これらの結果から、南極の鳥の糞から単離された大腸菌株では自然発生抗生物質耐性はまれで、海水の抗生物質耐性菌株はFildes半島の汚水処理施設からの処理排水と関わっていることが示唆された。

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