Journal Article Diagnostic Potential of Near-Infrared Raman Spectroscopy for Colon Cancer

Kazuhiro, Takabayashi  ,  Yoshihisa, Saida  ,  Toshiyuki, Enomoto  ,  Shinya, Kusachi  ,  Masahiro, Ando  ,  Hiro-o, Hamaguchi

1 ( 3 )  , pp.35 - 40 , 2015-09 , The Medical Society of Toho University
Original Article
Background: Raman spectroscopy measures the specific scattering (“Raman scattering”) of an irradiated target substance illuminated by laser light. The present study used near-infrared Raman spectroscopy to examine spectral differences between specimens of normal and cancerous tissues collected during colon cancer surgery. Methods: Normal and cancerous sites were randomly selected for each specimen, and Raman spectra were obtained using a Raman spectrometer with a 1064 nm excitation fiber optic probe attachment. Results: Although no pronounced marker bands specific to normal or cancerous sites were observed, spectra at normal sites had a higher baseline due to autofluorescence, as compared with cancerous sites. The focus was then changed to the 1003 cm-1 and 1447 cm-1 bands, and the band intensity ratio (1003 cm-1 band intensity)/(1447 cm-1 band intensity) was calculated for all spectra. The 1657 cm-1 band was also examined, and the band intensity ratio (1657 cm-1 band intensity)/(1447 cm-1 band intensity) was calculated. Using the two types of band intensity ratios, we obtained a two-dimensional plot, which made it possible to differentiate normal and cancerous sites with 91% sensitivity and 91% specificity. Conclusions: If the present method becomes applicable endoscopically, it will enable easy collection of Raman spectra of colon cancer biopsy samples. Thus, analysis of tissues based on information regarding their molecular structure is now feasible.

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