学術雑誌論文 Tumor-promoting function and prognostic significance of the RNA-binding protein T-cell intracellular antigen-1 in esophageal squamous cell carcinoma.

Hamada, Junichi  ,  Shoda, Katsutoshi  ,  Masuda, Kiyoshi  ,  Fujita, Yuji  ,  Naruto, Takuya  ,  Kohmoto, Tomohiro  ,  Miyakami, Yuko  ,  Watanabe, Miki  ,  Kudo, Yasusei  ,  Fujiwara, Hitoshi  ,  Ichikawa, Daisuke  ,  Otsuji, Eigo  ,  Imoto, Issei

7 ( 13 )  , pp.17111 - 17128 , 2016-03-06
ISSN:19492553
内容記述
T-cell intracellular antigen-1 (TIA1) is an RNA-binding protein involved in many regulatory aspects of mRNA metabolism. Here, we report previously unknown tumor-promoting activity of TIA1, which seems to be associated with its isoform-specific molecular distribution and regulation of a set of cancer-related transcripts, in esophageal squamous cell carcinoma (ESCC). Immunohistochemical overexpression of TIA1 ectopically localized in the cytoplasm of tumor cells was an independent prognosticator for worse overall survival in a cohort of 143 ESCC patients. Knockdown of TIA1 inhibited proliferation of ESCC cells. By exogenously introducing each of two major isoforms, TIA1a and TIA1b, only TIA1a, which was localized to both the nucleus and cytoplasm, promoted anchorage-dependent and anchorage-independent ESCC cell proliferation. Ribonucleoprotein immunoprecipitation, followed by microarray analysis or massive-parallel sequencing, identified a set of TIA1-binding mRNAs, including SKP2 and CCNA2. TIA1 increased SKP2 and CCNA2 protein levels through the suppression of mRNA decay and translational induction, respectively. Our findings uncover a novel oncogenic function of TIA1 in esophageal tumorigenesis, and implicate its use as a marker for prognostic evaluation and as a therapeutic target in ESCC.
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http://repo.lib.tokushima-u.ac.jp/files/public/11/110148/20170929142315687841/LID201705314001.pdf

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