学位論文 Functional characterization of tissue factor in von Willebrand factor-dependent thrombus formation under whole blood flow conditions.
全血流動下でのフォンビルブランド因子依存性血栓形成における組織因子の機能特性

Matsunari, Yasunori  ,  Sugimoto, Mitsuhiko  ,  Doi, Masaaki  ,  Matsui, Hideto  ,  Kawaguchi, Masahiko

104 ( 6 )  , pp.661 - 668 , 2016-12 , Springer Nature
ISSN:09255710
NII書誌ID(NCID):AA10797094
内容記述
Von Willebrand factor (VWF) plays an important role in mediating platelet adhesion and aggregation under high shear rate conditions. Such platelet aggregates are strengthened by fibrin-network formation triggered by tissue factor (TF). However, little is known about the role of TF in VWF-dependent thrombus formation under blood flow conditions. We evaluated TF in thrombus formation on immobilized VWF under whole blood flow conditions in an in vitro perfusion chamber system. Surface-immobilized TF amplified intra-thrombus fibrin generation significantly under both low and high shear flow conditions, while TF in sample blood showed no appreciable effects. Furthermore, immobilized TF enhanced VWF-dependent platelet adhesion and aggregation significantly under high shear rates. Neutrophil cathepsin G and elastase increased significantly intra-thrombus fibrin deposition on immobilized VWF–TF complex, suggesting the involvement of leukocyte inflammatory responses in VWF/TF-dependent mural thrombogenesis under these flow conditions. These results reveal a functional link between VWF and TF under whole blood flow conditions, in which surface-immobilized TF and VWF mutually contribute to mural thrombus formation, which is essential for normal hemostasis. By contrast, TF circulating in blood may be involved in systemic hypercoagulability, as seen in sepsis caused by severe microbial infection, in which neutrophil inflammatory responses may be active.
博士(医学)・乙第1388号・平成28年11月24日
© The Japanese Society of Hematology 2016
The original publication is available at www.springerlink.com
The final publication is available at Springer via http://dx.doi.org/10.1007/s12185-016-2086-z
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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3293/1/01_%e4%b9%991388%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3293/2/02_%e4%b9%991388%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3293/4/03_%e4%b9%991388%e6%9c%ac%e6%96%87.pdf

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