学位論文 Bone marrow stromal cell sheets may promote axonal regeneration and functional recovery with suppression of glial scar formation after spinal cord transection injury in rats.
骨髄間葉系細胞シートはラット脊髄離断損傷後にグリア瘢痕形成を抑制し、軸索再生と後肢運動機能改善を促進する。

Okuda, Akinori  ,  Horii-Hayashi, Noriko  ,  Sasagawa, Takayo  ,  Shimizu, Takamasa  ,  Shigematsu, Hideki  ,  Iwata, Eiichiro  ,  Morimoto, Yasuhiko  ,  Masuda, Keisuke  ,  Koizumi, Munehisa  ,  Akahane, Manabu  ,  Nishi, Mayumi  ,  Tanaka, Yasuhito

Epub ahead of print(2016 Nov 25)2016-11-25 , American Association of Neurological Surgeons
ISSN:15475654
NII書誌ID(NCID):AA11357949
内容記述
OBJECTIVE Transplantation of bone marrow stromal cells (BMSCs) is a theoretical potential as a therapeutic strategy in the treatment of spinal cord injury (SCI). Although a scaffold is sometimes used for retaining transplanted cells in damaged tissue, it is also known to induce redundant immunoreactions during the degradation processes. In this study, the authors prepared cell sheets made of BMSCs, which are transplantable without a scaffold, and investigated their effects on axonal regeneration, glial scar formation, and functional recovery in a completely transected SCI model in rats. METHODS BMSC sheets were prepared from the bone marrow of female Fischer 344 rats using ascorbic acid and were cryopreserved until the day of transplantation. A gelatin sponge (GS), as a control, or BMSC sheet was transplanted into a 2-mm-sized defect of the spinal cord at the T-8 level. Axonal regeneration and glial scar formation were assessed 2 and 8 weeks after transplantation by immunohistochemical analyses using anti-Tuj1 and glial fibrillary acidic protein (GFAP) antibodies, respectively. Locomotor function was evaluated using the Basso, Beattie, and Bresnahan scale. RESULTS The BMSC sheets promoted axonal regeneration at 2 weeks after transplantation, but there was no significant difference in the number of Tuj1-positive axons between the sheet- and GS-transplanted groups. At 8 weeks after transplantation, Tuj1-positive axons elongated across the sheet, and their numbers were significantly greater in the sheet group than in the GS group. The areas of GFAP-positive glial scars in the sheet group were significantly reduced compared with those of the GS group at both time points. Finally, hindlimb locomotor function was ameliorated in the sheet group at 4 and 8 weeks after transplantation. CONCLUSIONS The results of the present study indicate that an ascorbic acid-induced BMSC sheet is effective in the treatment of SCI and enables autologous transplantation without requiring a scaffold.
博士(医学)・甲第656号・平成28年11月24日
© Copyright 2016 American Association of Neurological Surgeons
The definitive version is available at " http://dx.doi.org/10.3171/2016.8.SPINE16250 "
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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3282/1/01_%e7%94%b2656%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3282/2/02_%e7%94%b2656%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3282/10/03%20%e7%94%b2656%e6%9c%ac%e6%96%87.pdf

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