学位論文 Promotion of osteogenesis and angiogenesis in vascularized tissue-engineered bone using osteogenic matrix cell sheets.
骨形成細胞シートは血管柄付き人工骨内での骨形成および血管形成を促進させる

Nakano, Kenichi  ,  Murata, Keiichi  ,  Omokawa, Shohei  ,  Akahane, Manabu  ,  Shimizu, Takamasa  ,  Kawamura, Kenji  ,  Kawate, Kenji  ,  Tanaka, Yasuhito

2016(掲載予定)2016 , Lippincott Williams & Wilkins
ISSN:00321052
NII書誌ID(NCID):AA00775696
内容記述
BACKGROUND: The regeneration of large, poorly vascularized bone defects remains a significant challenge. Although vascularized bone grafts promote osteogenesis, the required tissue harvesting causes problematic donor-site morbidity. Artificial bone substitutes are promising alternatives for regenerative medicine applications, but the incorporation of suitable cells and/or growth factors is necessary for their successful clinical application. The inclusion of vascular bundles can further enhance the bone-forming capability of bone substitutes by promoting tissue neovascularization. Little is known about how neovascularization occurs and how new bone extends within vascularized tissue-engineered bone, because no previous studies have used tissue-engineered bone to treat large, poorly vascularized defects. METHODS: In this study, the authors developed a novel vascularized tissue-engineered bone scaffold composed of osteogenic matrix cell sheets wrapped around vascular bundles within β-tricalcium phosphate ceramics. RESULTS: Four weeks after subcutaneous transplantation in rats, making use of the femoral vascular bundle, vascularized tissue-engineered bone demonstrated more angiogenesis and higher osteogenic potential than the controls. After vascularized tissue-engineered bone implantation, abundant vascularization and new bone formation were observed radially from the vascular bundle, with increased mRNA expression of alkaline phosphatase, bone morphogenetic protein-2, osteocalcin, and vascular endothelial growth factor-A. CONCLUSION: This novel method for preparing vascularized tissue-engineered bone scaffolds may promote the regeneration of large bone defects, particularly where vascularization has been compromised.
博士(医学)・甲第652号・平成28年3月15日
Copyright © 2016 American Society of Plastic Surgeons All rights reserved.
This is a non-final version of an article published in final form in "http://dx.doi.org/10.1097/PRS.0000000000002079"
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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3190/1/01%20%e7%94%b2652%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3190/2/02%20%e7%94%b2652%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf

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