学位論文 Use of Cryopreserved Osteogenic Matrix Cell Sheets for Bone Reconstruction.

Kura, Tomohiko  ,  Akahane, Manabu  ,  Shimizu, Takamasa  ,  Uchihara, Yoshinobu  ,  Tohma, Yasuaki  ,  Morita, Yusuke  ,  Koizumi, Munehisa  ,  Kawate, Kenji  ,  Tanaka, Yasuhito

6 ( 1 )  , pp.13 - 23 , 2016-01 , Scientific Research Publishing Inc.
ISSN:21616760
内容記述
Skeletal diseases, such as nonunion and osteonecrosis, are now treatable with tissue engineering techniques. Single cell sheets called osteogenic matrix cell sheets (OMCSs) grown from cultured bone marrow-derived mesenchymal stem cells show high osteogenic potential; however, long preparation times currently limit their clinical application. Here, we report a cryopreservation OMCS transplantation method that shortens OMCS preparation time. Cryopreserved rat OMCSs were prepared using slow- and rapid-freezing methods, thawed, and subsequently injected scaffold-free into subcutaneous sites. Rapid- and slow-frozen OMCSs were also transplanted directly to the femur bone at sites of injury. Slow-freezing resulted in higher cell viability than rapid freezing, yet all two cryopreservation methods yielded OMCSs that survived and formed bone tissue. In the rapid- and slow-freezing groups, cortical gaps were repaired and bone continuity was observed within 6 weeks of OMCS transplantation. Moreover, while no significant difference was found in osteocalcin expression between the three experimental groups, the biomechanical strength of femurs treated with slow-frozen OMCSs was significantly greater than those of non-transplant at 6 weeks post-injury. Collectively, these data suggest that slow-frozen OMCSs have superior osteogenic potential and are better suited to produce a mineralized matrix and repair sites of bone injury.
博士(医学)・甲第650号・平成28年3月15日
Copyright © 2016 by authors and Scientific Research Publishing Inc.This work is licensed under the Creative Commons Attribution International License (CC BY).http://creativecommons.org/licenses/by/4.0/
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http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3187/1/01%20%e7%94%b2650%e6%9c%ac%e6%96%87%e3%81%ae%e8%a6%81%e6%97%a8.pdf

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3187/2/02%20%e7%94%b2650%e5%af%a9%e6%9f%bb%e8%a6%81%e6%97%a8.pdf

http://ginmu.naramed-u.ac.jp/dspace/bitstream/10564/3187/3/03%20%e7%94%b2650%e6%9c%ac%e6%96%87.pdf

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